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Production of Membrane Proteins for NMR Studies Using the Condensed Single Protein Production (cSPP) System
In the Single Protein Production (SPP) method, all E. coli cellular mRNAs are eliminated by the induction of MazF, an ACA-specific mRNA interferase. When an mRNA for a membrane protein, engineered to have no ACA sequences without altering its amino acid sequence, is induced in the MazF-induced cells...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2923930/ https://ncbi.nlm.nih.gov/pubmed/19856129 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-009-9072-0 |
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