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In-vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1
We present the in-vivo biosynthesis of wild-type sunflower trypsin inhibitor 1 (SFTI-1) inside E. coli cells using an intramolecular native chemical ligation in combination with a modified protein splicing unit. SFTI-1 is a small backbone cyclized polypeptide with a single disulfide bridge. A small...
Tallennettuna:
| Päätekijät: | , , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2009
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2936557/ https://ncbi.nlm.nih.gov/pubmed/19685144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00726-009-0338-4 |
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