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Polar Residues in Transmembrane Helices can Decrease Electrophoretic Mobility in Polyacrylamide Gels Without Causing Helix Dimerization

There are only a few available methods to study lateral interactions and self assembly of transmembrane helices. One of the most frequently used methods is sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) which can report on strong interactions between peptides in SDS solution. He...

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Autors principals: Walkenhorst, William F., Merzlyakov, Mikhail, Hristova, Kalina, Wimley, William C.
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935849/
https://ncbi.nlm.nih.gov/pubmed/19265670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2009.02.017
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