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Stability of the Neurotensin Receptor NTS1 Free in Detergent Solution and Immobilized to Affinity Resin

BACKGROUND: Purification of recombinant membrane receptors is commonly achieved by use of an affinity tag followed by an additional chromatography step if required. This second step may exploit specific receptor properties such as ligand binding. However, the effects of multiple purification steps o...

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Main Authors: White, Jim F., Grisshammer, Reinhard
格式: Artigo
語言:Inglês
出版: Public Library of Science 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935352/
https://ncbi.nlm.nih.gov/pubmed/20830205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0012579
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