Isolation from Ochrobactrum anthropi of a Novel Class II 5-Enopyruvylshikimate-3-Phosphate Synthase with High Tolerance to Glyphosate

Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was e...

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Hlavní autoři: Tian, Yong-Sheng, Xiong, Ai-Sheng, Xu, Jing, Zhao, Wei, Gao, Feng, Fu, Xiao-Yan, Xu, Hu, Zheng, Jian-Li, Peng, Ri-He, Yao, Quan-Hong
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2010
Témata:
Enzymology and Protein Engineering
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935038/
https://ncbi.nlm.nih.gov/pubmed/20601515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00770-10
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