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Combining Isoelectric Point-Based Fractionation, Liquid Chromatography and Mass Spectrometry to Improve Peptide Detection and Protein Identification

The off-line coupling of an isoelectric trapping device termed membrane separated wells for isoelectric focusing and trapping (MSWIFT) to mass spectrometry-based proteomic studies is described. The MSWIFT is a high capacity, high-throughput, mass spectrometry compatible, isoelectric trapping device...

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Hlavní autoři: Cologna, Stephanie M., Russell, William K., Lim, Peniel J., Vigh, Gyula, Russell, David H.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2927729/
https://ncbi.nlm.nih.gov/pubmed/20537905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2010.04.010
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