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Effect of Molecular Crowding and Ionic Strength on the Isothermal Hybridization of Oligonucleotides
The isothermal hybridization of complimentary oligonucleotides, 15-mer, 25-mer, 35-mer, and a molecular beacon, was investigated under varying conditions of molecular crowding and ionic strength, using hypochromicity to follow strand pairing and polyethylene glycol as a crowding agent. Thermodynamic...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2925268/ https://ncbi.nlm.nih.gov/pubmed/20701389 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp103213w |
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