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Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4

Alginate epimerases are large multidomain proteins capable of epimerising C5 on β-d-mannuronic acid (M) turning it into α-l-guluronic acid (G) in a polymeric alginate. Azotobacter vinelandii secretes a family of seven epimerases, each of which is capable of producing alginates with characteristic G...

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Detalhes bibliográficos
Main Authors: Buchinger, Edith, Aachmann, Finn L, Aranko, A Sesilja, Valla, Svein, Skjåk-BræK, Gudmund, Iwaï, Hideo, Wimmer, Reinhard
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2923506/
https://ncbi.nlm.nih.gov/pubmed/20552686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.432
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