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Biochemical analysis of the processive mechanism for epimerization of alginate by mannuronan C-5 epimerase AlgE4

The enzymes mannuronan C-5 epimerases catalyse the in-chain epimerisation of β-D-mannuronic acid to α-L-guluronic acid in the last step of alginate biosynthesis. The recombinant C-5 epimerase AlgE4, encoded by the soil bacteria Azotobacter vinelandii and expressed in Escherichia coli, exhibits a non...

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Autors principals: Campa, Cristiana, Holtan, Synnøve, Nilsen, Nadra, Bjerkan, Tonje M., Stokke, Bjørn T., SKJåK-BRæK, Gudmund
Format: Artigo
Idioma:Inglês
Publicat: Portland Press Ltd. 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133773/
https://ncbi.nlm.nih.gov/pubmed/15032753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031265
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