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Engineering of a wheat germ expression system to provide compatibility with a high throughput pET-based cloning platform
Wheat germ cell-free methods provide an important approach for the production of eukaryotic proteins. We have developed a protein expression vector for the TNT(®) SP6 High-Yield Wheat Germ Cell-Free (TNT WGCF) expression system (Promega) that is also compatible with our T7-based Escherichia coli int...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2921493/ https://ncbi.nlm.nih.gov/pubmed/20574660 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-010-9093-8 |
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