The High-Throughput Protein Sample Production Platform of the Northeast Structural Genomics Consortium

We describe the core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) and outline the strategies used for producing high-quality protein samples. The platform is centered on the cloning, expression and purification of 6X-His-tagged proteins using T7-based Escherichi...

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Detalhes bibliográficos
Main Authors: Xiao, Rong, Anderson, Stephen, Aramini, James, Belote, Rachel, Buchwald, William A., Ciccosanti, Colleen, Conover, Ken, Everett, John K., Hamilton, Keith, Huang, Yuanpeng Janet, Janjua, Haleema, Jiang, Mei, Kornhaber, Gregory J., Lee, Dong Yup, Locke, Jessica Y., Ma, Li-Chung, Maglaqui, Melissa, Mao, Lei, Mitra, Saheli, Patel, Dayaban, Rossi, Paolo, Sahdev, Seema, Sharma, Seema, Shastry, Ritu, Swapna, G.V.T., Tong, Saichu N., Wang, Dongyan, Wang, Huang, Zhao, Li, Montelione, Gaetano T., Acton, Thomas B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4110633/
https://ncbi.nlm.nih.gov/pubmed/20688167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2010.07.011
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