Rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction.
An 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of MA-104 cells were treated with 50 microgram of trypsin per ml and hydroextracted overnight (4 degrees C) with polyethylene glycol 6,000. The concentrate was resuspended in 8 to 10 ml of tryptose phosphate broth a...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6257163/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.40.6.1133-1135.1980 |
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