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Rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction.

An 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of MA-104 cells were treated with 50 microgram of trypsin per ml and hydroextracted overnight (4 degrees C) with polyethylene glycol 6,000. The concentrate was resuspended in 8 to 10 ml of tryptose phosphate broth a...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Ramia, S, Sattar, S A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1980
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291732/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6257163/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.40.6.1133-1135.1980
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