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Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae

The rapid and inexpensive production of high-quality eukaryotic proteins in recombinant form still remains a challenge in structural biology. Here, a protein-expression system based on the protozoan Leishmania tarentolae was used to produce human Cu/Zn superoxide dismutase (SOD1) in recombinant form...

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Detalhes bibliográficos
Main Authors: Gazdag, Emerich Mihai, Cirstea, Ion Cristian, Breitling, Reinhard, Lukeš, Julius, Blankenfeldt, Wulf, Alexandrov, Kirill
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2917280/
https://ncbi.nlm.nih.gov/pubmed/20693657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110019330
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