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Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes

A real-time quantitative PCR-based detection assay targeting the dnaJ gene (encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 10(4) CFU per cm(2) of coral tissue. Moreover, inhibitio...

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Autors principals: Pollock, F. Joseph, Morris, Pamela J., Willis, Bette L., Bourne, David G.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2916481/
https://ncbi.nlm.nih.gov/pubmed/20525865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00330-10
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