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Detection of Vibrio parahaemolyticus in Shellfish by Use of Multiplexed Real-Time PCR with TaqMan Fluorescent Probes

We developed a multiplexed real-time PCR assay using four sets of gene-specific oligonucleotide primers and four TaqMan probes labeled with four different fluorophores in a single reaction for detection of total and pathogenic Vibrio parahaemolyticus, including the pandemic O3:K6 serotype in oysters...

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Detalhes bibliográficos
Main Authors: Ward, Linda N., Bej, Asim K.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1393209/
https://ncbi.nlm.nih.gov/pubmed/16517652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.3.2031-2042.2006
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