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Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol.

Polyethylene glycol (PEG) dehydrogenase in crude extracts of a PEG 20,000-utilizing mixed culture was purified 24 times by precipitation with ammonium sulfate, solubilization with laurylbetaine, and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite, and Sephadex G-200. The purified e...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Kawai, F, Kimura, T, Tani, Y, Yamada, H, Kurachi, M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291647/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6999995/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.40.4.701-705.1980
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