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Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol.

Polyethylene glycol (PEG) dehydrogenase in crude extracts of a PEG 20,000-utilizing mixed culture was purified 24 times by precipitation with ammonium sulfate, solubilization with laurylbetaine, and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite, and Sephadex G-200. The purified e...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kawai, F, Kimura, T, Tani, Y, Yamada, H, Kurachi, M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1980
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC291647/
https://ncbi.nlm.nih.gov/pubmed/6999995
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