Acetylene Reduction Assay for Nitrogenase Activity: Gas Chromatographic Determination of Ethylene Per Sample in Less Than One Minute

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatogra...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	David, K. A. V., Apte, S. K., Banerji, A., Thomas, Joseph
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1980
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291480/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16345570/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.39.5.1078-1080.1980
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