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Acetylene Reduction Assay for Nitrogenase Activity: Gas Chromatographic Determination of Ethylene Per Sample in Less Than One Minute

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatogra...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: David, K. A. V., Apte, S. K., Banerji, A., Thomas, Joseph
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1980
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291480/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16345570/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.39.5.1078-1080.1980
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