Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymes

We describe the fusion of enhanced green fluorescent protein to the C-terminus of the HsdS DNA sequence-specificity subunit of the Type I DNA modification methyltransferase M.EcoKI. The fusion expresses well in vivo and assembles with the two HsdM modification subunits. The fusion protein functions...

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Bibliographic Details
Main Authors: Chen, Kai, Roberts, Gareth A., Stephanou, Augoustinos S., Cooper, Laurie P., White, John H., Dryden, David T.F.
Format: Artigo
Language:Inglês
Published: Academic Press 2010
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2914225/
https://ncbi.nlm.nih.gov/pubmed/20599730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.06.069
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