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Mutagenesis of Klebsiella aerogenes UreG to Probe Nickel Binding and Interactions with Other Urease-Related Proteins

UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreG(Str)) and selected site-directed variants of UreG(Str) were constructed for studying the in vivo effects on urease activation in recombinant Escherichia coli...

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Main Authors: Boer, Jodi L., Quiroz-Valenzuela, Soledad, Anderson, Kimberly L., Hausinger, Robert P.
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2912431/
https://ncbi.nlm.nih.gov/pubmed/20533838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1004987
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