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Mutagenesis of Klebsiella aerogenes UreG to Probe Nickel Binding and Interactions with Other Urease-Related Proteins
UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreG(Str)) and selected site-directed variants of UreG(Str) were constructed for studying the in vivo effects on urease activation in recombinant Escherichia coli...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2912431/ https://ncbi.nlm.nih.gov/pubmed/20533838 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1004987 |
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