Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts

INTRODUCTION: Rheumatoid arthritis synovial fibroblasts (RASF) are key players in synovial pathophysiology and are therefore examined extensively in various experimental approaches. We evaluated, whether passaging during culture and freezing has effects on gene expression and cell proliferation. MET...

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Autori principali: Neumann, Elena, Riepl, Birgit, Knedla, Anette, Lefèvre, Stephanie, Tarner, Ingo H, Grifka, Joachim, Steinmeyer, Jurgen, Schölmerich, Jurgen, Gay, Steffen, Müller-Ladner, Ulf
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2010
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2911867/
https://ncbi.nlm.nih.gov/pubmed/20462438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/ar3010
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