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Deletion of Switch 3 Results in an Archaeal RNA Polymerase That Is Defective in Transcript Elongation

Switch 3 is a polypeptide loop conserved in all multisubunit DNA-dependent RNA polymerases (RNAPs) that extends into the main cleft of the RNAP and contacts each base in a nascent transcript as that base is released from the internal DNA-RNA hybrid. Plasmids have been constructed and transformed int...

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Autors principals: Santangelo, Thomas J., Reeve, John N.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2911337/
https://ncbi.nlm.nih.gov/pubmed/20511223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.094565
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