Distance Variations between Active Sites of H(+)-Pyrophosphatase Determined by Fluorescence Resonance Energy Transfer

Homodimeric H(+)-pyrophosphatase (H(+)-PPase; EC 3.6.1.1) is a unique enzyme playing a pivotal physiological role in pH homeostasis of organisms. This novel H(+)-PPase supplies energy at the expense of hydrolyzing metabolic byproduct, pyrophosphate (PP(i)), for H(+) translocation across membrane. Th...

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Main Authors: Huang, Yun-Tzu, Liu, Tseng-Huang, Chen, Yen-Wei, Lee, Chien-Hsien, Chen, Hsueh-Hua, Huang, Tsu-Wei, Hsu, Shen-Hsing, Lin, Shih-Ming, Pan, Yih-Jiuan, Lee, Ching-Hung, Hsu, Ian C., Tseng, Fan-Gang, Fu, Chien-Chung, Pan, Rong-Long
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2010
Fag:
Bioenergetics
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2911311/
https://ncbi.nlm.nih.gov/pubmed/20511234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.134916
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