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In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
In vitro reconstitution of a biological complex or process normally involves assembly of multiple individually purified protein components. Here we present a strategy that couples expression and assembly of multiple gene products with functional detection in an in vitro reconstituted protein synthes...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2010
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2910072/ https://ncbi.nlm.nih.gov/pubmed/20457746 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq377 |
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