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Centrifugal Methods and Devices for Rapid In-Gel Digestion of Proteins

Modern proteomic research frequently relies upon separation of proteins in a polyacrylamide gel matrix followed by in-gel enzymatic digestion and extraction of peptides for subsequent analysis by mass spectrometry. In this work, we propose a novel semi-automated method of mechanical processing of ge...

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Detalhes bibliográficos
Main Authors: Lazarev, Alexander V., Rejtar, Tomas, Dai, Shujia, Karger, Barry L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2907145/
https://ncbi.nlm.nih.gov/pubmed/19309014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200800474
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