A new technique for obtaining whole pathogen transcriptomes from infected host tissues

We propose a novel experimental approach based on coincidence cloning for analyzing sequences of bacterial intracellular pathogens specifically transcribed in affected tissues. Co-denaturation and co-renaturation of excess bacterial genomic DNA with the cDNA prepared on total RNA of the infected tis...

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Main Authors: Azhikina, Tatyana L., Skvortsov, Timofey A., Radaeva, Tatyana V., Mardanov, Andrey V., Ravin, Nikolay V., Apt, Alexander S., Sverdlov, Eugene D.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2905682/
https://ncbi.nlm.nih.gov/pubmed/20359298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113350
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