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A new technique for obtaining whole pathogen transcriptomes from infected host tissues
We propose a novel experimental approach based on coincidence cloning for analyzing sequences of bacterial intracellular pathogens specifically transcribed in affected tissues. Co-denaturation and co-renaturation of excess bacterial genomic DNA with the cDNA prepared on total RNA of the infected tis...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2905682/ https://ncbi.nlm.nih.gov/pubmed/20359298 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113350 |
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