Pitfalls and solutions in assaying anandamide transport in cells

Nonspecific binding of anandamide to plastic exhibits many features that could be mistaken as biological processes, thereby representing an important source of conflicting data on the uptake and release of this lipophilic substance. Herein, we propose an improved method to assay anandamide transport...

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Bibliographic Details
Main Authors: Oddi, Sergio, Fezza, Filomena, Catanzaro, Giuseppina, De Simone, Chiara, Pucci, Mariangela, Piomelli, Daniele, Finazzi-Agrò, Alessandro, Maccarrone, Mauro
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2010
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2903826/
https://ncbi.nlm.nih.gov/pubmed/20447929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D004176
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