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Mitochondrial Phosphoproteome Revealed by an Improved IMAC Method and MS/MS/MS

IMAC in combination with mass spectrometry is a promising approach for global analysis of protein phosphorylation. Nevertheless this approach suffers from two shortcomings: inadequate efficiency of IMAC and poor fragmentation of phosphopeptides in the mass spectrometer. Here we report optimization o...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lee, Jaeick, Xu, Yingda, Chen, Yue, Sprung, Robert, Kim, Sung Chan, Xie, Shanhai, Zhao, Yingming
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2007
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2902983/
https://ncbi.nlm.nih.gov/pubmed/17208939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M600218-MCP200
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