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Ensemble Methods for Monitoring Enzyme Translocation along Single Stranded Nucleic Acids
We review transient kinetic methods developed to study the mechanism of translocation of nucleic acid motor proteins. One useful stopped-flow fluorescence method monitors arrival of the translocase at the end of a fluorescently labeled nucleic acid. When conducted under single-round conditions the t...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2900532/ https://ncbi.nlm.nih.gov/pubmed/20371288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.03.010 |
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