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Characterization, crystallization and preliminary X-­ray analysis of the adhesive domain of SdrE from Staphylococcus aureus

The adhesive domain of SdrE from Staphylococcus aureus was recombinantly expressed in Escherichia coli. The purified protein was identified by SDS–PAGE and MALDI–TOF MS. The protein was crystallized using the vapour-diffusion method in hanging-drop mode with PEG 8000 as the primary precipitating age...

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Bibliografiske detaljer
Main Authors: Xiang, Hua, Gao, Fangfang, Wang, Dacheng, Liu, Jing, Hu, Jia, Zhang, Liqing, Li, Shentao, Deng, Xuming
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2898480/
https://ncbi.nlm.nih.gov/pubmed/20606292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110020907
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