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Glutamate 90 at the Luminal Ion Gate of Sarcoplasmic Reticulum Ca(2+)-ATPase Is Critical for Ca(2+) Binding on Both Sides of the Membrane
The roles of Ser(72), Glu(90), and Lys(297) at the luminal ends of transmembrane helices M1, M2, and M4 of sarcoplasmic reticulum Ca(2+)-ATPase were examined by transient and steady-state kinetic analysis of mutants. The dependence on the luminal Ca(2+) concentration of phosphorylation by P(i) (“Ca(...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2898329/ https://ncbi.nlm.nih.gov/pubmed/20421308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.116459 |
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