Split Renilla Luciferase Protein Fragment-assisted Complementation (SRL-PFAC) to Characterize Hsp90-Cdc37 Complex and Identify Critical Residues in Protein/Protein Interactions

Hsp90 requires cochaperone Cdc37 to load its clients to the Hsp90 superchaperone complex. The purpose of this study was to utilize split Renilla luciferase protein fragment-assisted complementation (SRL-PFAC) bioluminescence to study the full-length human Hsp90-Cdc37 complex and to identity critical...

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Auteurs principaux: Jiang, Yiqun, Bernard, Denzil, Yu, Yanke, Xie, Yehua, Zhang, Tao, Li, Yanyan, Burnett, Joseph P., Fu, Xueqi, Wang, Shaomeng, Sun, Duxin
Format: Artigo
Langue:Inglês
Publié: American Society for Biochemistry and Molecular Biology 2010
Sujets:
Protein Structure and Folding
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2898314/
https://ncbi.nlm.nih.gov/pubmed/20413594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.103390
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