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Biases in Illumina transcriptome sequencing caused by random hexamer priming
Generation of cDNA using random hexamer priming induces biases in the nucleotide composition at the beginning of transcriptome sequencing reads from the Illumina Genome Analyzer. The bias is independent of organism and laboratory and impacts the uniformity of the reads along the transcriptome. We pr...
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2010
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2896536/ https://ncbi.nlm.nih.gov/pubmed/20395217 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq224 |
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