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PCRTiler: automated design of tiled and specific PCR primer pairs

Efficiency and specificity of PCR amplification is dependent on several parameters, such as amplicon length, as well as hybridization specificity and melting temperature of primer oligonucleotides. Primer design is thus of critical importance for the success of PCR experiments, but can be a time-con...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Gervais, Alain L., Marques, Maud, Gaudreau, Luc
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2896098/
https://ncbi.nlm.nih.gov/pubmed/20519202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq485
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