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Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

BACKGROUND: Cross-species primers have been used with moderate success to address a variety of questions concerning genome structure, evolution, and gene function. However, the factors affecting their success have never been adequately addressed, particularly with respect to producing a consistent m...

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Detalhes bibliográficos
Main Authors: Housley, Donna JE, Zalewski, Zachary A, Beckett, Stephanie E, Venta, Patrick J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635982/
https://ncbi.nlm.nih.gov/pubmed/17029642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-7-253
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