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Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts

Development of a transgenic porcine biomedical research model requires effective delivery of DNA into the donor cell followed by selection of genetically modified somatic cell lines to be used for nuclear transfer. The objective of the current study was two-fold: 1) to compare the effectiveness of a...

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Bibliographic Details
Main Authors: Ross, Jason W., Whyte, Jeffrey J., Zhao, Jianguo, Samuel, Melissa, Wells, Kevin D., Prather, Randall S.
Format: Artigo
Language:Inglês
Published: 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2894267/
https://ncbi.nlm.nih.gov/pubmed/19937273
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11248-009-9345-1
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