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Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts
Development of a transgenic porcine biomedical research model requires effective delivery of DNA into the donor cell followed by selection of genetically modified somatic cell lines to be used for nuclear transfer. The objective of the current study was two-fold: 1) to compare the effectiveness of a...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2009
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2894267/ https://ncbi.nlm.nih.gov/pubmed/19937273 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11248-009-9345-1 |
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