A Real-Time Polymerase Chain Reaction Assay for Rapid, Sensitive, and Specific Quantification of the JAK2V617F Mutation Using a Locked Nucleic Acid-Modified Oligonucleotide

The JAK2V617F mutation has emerged as an essential molecular determinant of myeloproliferative neoplasms (MPNs). The aim of this study was to evaluate the analytical and clinical performances of a real-time PCR (qPCR) assay using a combination of hydrolysis probes and a wild-type blocking oligonucle...

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Detalhes bibliográficos
Main Authors: Denys, Barbara, El Housni, Hakim, Nollet, Friedel, Verhasselt, Bruno, Philippé, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2010
Assuntos:
Regular Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2893637/
https://ncbi.nlm.nih.gov/pubmed/20431031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090137
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