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SPUD qPCR Assay Confirms PREXCEL-Q Software’s Ability to Avoid qPCR Inhibition

Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. W...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Gallup, J.M., Sow, F.B., Van Geelen, A., Ackermann, M.R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2891329/
https://ncbi.nlm.nih.gov/pubmed/19776427
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