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Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamica...

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Detalhes bibliográficos
Main Authors: Gallup, Jack M., Ackermann, Mark R.
Formato: Artigo
Idioma:Inglês
Publicado em: Biological Procedures Online 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1592462/
https://ncbi.nlm.nih.gov/pubmed/17033699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo122
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