Cloning of NruI and Sbo13I restriction and modification sstems in E. coli and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases

BACKGROUND: NruI and Sbo13I are restriction enzyme isoschizomers with the same recognition sequence 5' TCG↓CGA 3' (cleavage as indicated↓). Here we report the cloning of NruI and Sbo13I restriction-modification (R-M) systems in E. coli. The NruI restriction endonuclease gene (nruIR) was cl...

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Détails bibliographiques
Auteurs principaux: Zhu, Zhenyu, Pedamallu, Chandra Sekhar, Fomenkov, Alexey, Benner, Jack, Xu, Shuang-yong
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2010
Sujets:
Research article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2890505/
https://ncbi.nlm.nih.gov/pubmed/20497557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-139
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