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Selection of optimal reference genes for normalization in quantitative RT-PCR
BACKGROUND: Normalization in real-time qRT-PCR is necessary to compensate for experimental variation. A popular normalization strategy employs reference gene(s), which may introduce additional variability into normalized expression levels due to innate variation (between tissues, individuals, etc)....
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| Hauptverfasser: | , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2010
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2889935/ https://ncbi.nlm.nih.gov/pubmed/20470420 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-11-253 |
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