Selection of optimal reference genes for normalization in quantitative RT-PCR

BACKGROUND: Normalization in real-time qRT-PCR is necessary to compensate for experimental variation. A popular normalization strategy employs reference gene(s), which may introduce additional variability into normalized expression levels due to innate variation (between tissues, individuals, etc)....

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Autores principales: Chervoneva, Inna, Li, Yanyan, Schulz, Stephanie, Croker, Sean, Wilson, Chantell, Waldman, Scott A, Hyslop, Terry
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2010
Materias:
Methodology article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2889935/
https://ncbi.nlm.nih.gov/pubmed/20470420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-11-253
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