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Direct Dynamic Protein-Affinity Selection Mass-Spectrometry

A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERα-LBD). In-solution incubation is performed of the analyte and the His-tagged ERα-LBD. The bound complex is immobilized on a nickel-loaded protein-af...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Jonker, Niels, Lingeman, Henk, Irth, Hubertus
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Vieweg Verlag 2010
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2889285/
https://ncbi.nlm.nih.gov/pubmed/20628447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1365/s10337-010-1586-x
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