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Direct Dynamic Protein-Affinity Selection Mass-Spectrometry
A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERα-LBD). In-solution incubation is performed of the analyte and the His-tagged ERα-LBD. The bound complex is immobilized on a nickel-loaded protein-af...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Vieweg Verlag
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2889285/ https://ncbi.nlm.nih.gov/pubmed/20628447 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1365/s10337-010-1586-x |
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