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Catalytic Mechanism of Heparinase II Investigated by Site-directed Mutagenesis and the Crystal Structure with Its Substrate

Heparinase II (HepII) is an 85-kDa dimeric enzyme that depolymerizes both heparin and heparan sulfate glycosaminoglycans through a β-elimination mechanism. Recently, we determined the crystal structure of HepII from Pedobacter heparinus (previously known as Flavobacterium heparinum) in complex with...

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Bibliografiske detaljer
Main Authors: Shaya, David, Zhao, Wenjing, Garron, Marie-Line, Xiao, Zhongping, Cui, Qizhi, Zhang, Zhenqing, Sulea, Traian, Linhardt, Robert J., Cygler, Miroslaw
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2010
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2888417/
https://ncbi.nlm.nih.gov/pubmed/20404324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.101071
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