Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNA(Tyr) pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase–tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase (TyrRS)–...

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Autores principales: Iraha, Fumie, Oki, Kenji, Kobayashi, Takatsugu, Ohno, Satoshi, Yokogawa, Takashi, Nishikawa, Kazuya, Yokoyama, Shigeyuki, Sakamoto, Kensaku
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2010
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2887954/
https://ncbi.nlm.nih.gov/pubmed/20159998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq080
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