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Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNA(Tyr) pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion
Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase–tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase (TyrRS)–...
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| 主要な著者: | , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2010
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2887954/ https://ncbi.nlm.nih.gov/pubmed/20159998 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq080 |
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